Fig. 7.

Parmodulins are protective in the setting of thromboinflammation. (A) Mice were injected i.v. with vehicle or PM2 (5–10 mg/kg) 2.5 h before 10 mg/kg LPS administration i.p. and with an additional bolus 0.5 h after LPS exposure. Thrombus formation was induced by endothelial laser injury in cremaster arterioles 1–3 h following LPS injection. Platelet (red) and fibrin (green) accumulation were monitored for 180 s using Dylight 647-labeled antiplatelet antibody (CD42b) and Dylight 488-labeled antifibrin antibody (59D8). Representative binarized images from a single thrombus are shown for vehicle, PM2, and LPS and PM2 followed by LPS. (B and C) Median integrated platelet and fibrin fluorescent intensities following laser injury were calculated for all thrombi in vehicle (n = 37), PM2 (n = 37), LPS (n = 30), and PM2 followed by LPS (n = 38) experiments. (Magnification: 60×.) (B) Median integrated platelet fluorescent intensities are indicated for vehicle (black), PM2 (gray), LPS alone (red), PM2 followed by LPS (pink). (C) Median integrated platelet fluorescent intensities are indicated for vehicle (black), PM2 (gray), LPS alone (dark green), and PM2 followed by LPS (light green). A Kruskal–Wallis test with multiple comparisons was used. *P < 0.05. **P < 0.01.