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. 2018 Jan 16;115(5):E944–E953. doi: 10.1073/pnas.1719960115

Fig. 3.

Fig. 3.

Effects of long-term incubation of TSC2+/− cells with isoproterenol (ISO) on phosphorylation of S6 and P70. (A) TSC2+/− cells were incubated with 1 μM ISO for the indicated time before analysis by Western blotting with indicated antibodies and densitometric quantification. *P < 0.01 vs. control (Con). (B) After a 72-h knockdown of TSC2, cells were incubated with 1 μM ISO for the indicated time before analysis of indicated proteins by Western blotting and densitometric quantification. *P < 0.005 vs. TSC2 siRNA. Cells were incubated with DMSO or sirolimus (Siro) for 3 d, with or without ISO (C) or with ISO for 1 h each day for 3 d (E) before analysis by Western blotting with indicated antibodies and densitometric quantification. *P < 0.01 vs. Con; **P < 0.005 vs. DMSO; ***P < 0.01 vs. Siro. After a 72-h knockdown of TSC2, cells were incubated with DMSO or 200 nM Siro for 3 d, with or without 1 μM ISO (D) or with 1 μM ISO for 1 h each day for 3 d (F) before analysis of indicated proteins by Western blotting and densitometric quantification. *P < 0.01 vs. Con + nontargeted (NT) siRNA; **P < 0.01 vs. Con + TSC2 siRNA; #P < 0.01 vs. DMSO + TSC2 siRNA; ***P < 0.01 vs. Siro + TSC2 siRNA. Fig. S4 shows the effects on 4EBP1 and ULK1.