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. 2018 Jan 16;115(5):E944–E953. doi: 10.1073/pnas.1719960115

Fig. 4.

Fig. 4.

Effects of isoproterenol (ISO) or 8-Br-cAMP on phosphorylation of S6 in cells cultured from explanted lungs of patients with LAM who underwent transplantation. (A and C) Cells grown from LAM lung explants were incubated for 1 h with DMSO or 200 nM sirolimus (Siro), followed by 1 h with 1 μM ISO or 1 mM cAMP before analysis of indicated proteins by Western blotting and densitometric quantification. *P < 0.05 vs. control (Con); **P < 0.01 vs. DMSO; ***P < 0.01 vs. Siro. (B and D) Cells were incubated with DMSO or 200 nM Siro for 3 d, with or without 1 μM ISO or 1 mM cAMP, before analysis by Western blotting with indicated antibodies. Fig. S7 shows the effects on 4EBP1 and ULK1.