Figure 3. CBCs Are Induced following RV Infection.

(A) qRT-PCR results showed upregulation of all CBC markers and some reserve ISC markers.

(B) Schematic of Lgr5^GFPCreERT mice. Cells expressing LGR5 are green.

(C) Representative flow cytometry analysis on control- and RV-infected Lgr5^GFPCreERTmice. Quantification of GFP⁺ cells using flow cytometry analysis on crypt-enriched epithelial preparations.

(D) Representative confocal images of Lgr5^GFPCreERT mice following control- and RV-infection. White arrowheads denote GFP⁺ cells.

(E) Representative light microscopy images of OLFM4 immunohistochemistry staining in control- and RV-infected animals.

(F) Schematic of Bmi1^CreERT;R26^mTmG mice.

(G) Representative flow cytometry analysis on control- and RV-infected Bmi1^CreERT;R26^mTmG mice. Quantification of BMI1-mGFP⁺ cells using flow cytometry analysis on crypt-enriched epithelial preparations.

(H) Representative confocal images of BMI1-mGFP⁺ mice following control- and RV-infection. White arrows denote BMI1-mGFP⁺ cells.

Scale bars, 50 μm.