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. Author manuscript; available in PMC: 2018 Feb 5.
Published in final edited form as: Nat Med. 2017 May 8;23(6):768–774. doi: 10.1038/nm.4329

Figure 3.

Figure 3

Chronic sleep deprivation but not chronic sleep fragmentation progressively increases pain sensitivity. (a) Left, latency to withdrawal from noxious heat contact (52 °C hot plate). Right, mechanical sensitivity measured as the number of brisk withdrawals from ten stimulations with von Frey filaments of different forces that were applied to the plantar surface of the hindpaw. Mice were tested at baseline, CSD1d, CSD3d, CSD5d and on recovery (n = 12 mice). (b) Left, schematic representation of the CSF protocol and a representative EEG trace of fragmented sleep. A platform below the cage briefly (10–20 ms) moved upward at a random intensity every minute from 7:00 to 19:00 over five consecutive days. Mice were tested for sensory responsiveness at baseline and CSF5d. Middle, latency to withdrawal from heat contact (52 °C hot plate). Right, mechanical sensitivity. n = 8 mice; nonsignificant Student’s t-test. Scale bar, 10 s. Data are presented as means ± s.e.m. Circles overlaid upon the bar in histograms represent data from each individual animal. *P < 0.05, in comparison to baseline. For complete statistical analyses (post hoc test, within-subjects or inter-subjects comparison, effect size), please refer to Supplementary Table 1.