Fig. 3.

Macitentan treatment improves vascular function in thoracic aortae. Graphs show cumulative dose response curves to increasing concentrations of acetylcholine (a) or sodium nitroprusside (b) in thoracic aortae pre-contracted with 10 µM phenylephrine. Data are expressed as mean ± SEM and presented as a % of endothelium-dependent (a) or -independent (b) relaxation, n = 4. *P < 0.05, ***P < 0.001. Statistical differences between treated and untreated HFD-fed mice were assessed by two-way analysis of variance with Bonferroni post-hoc analysis.