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. 2018 Feb 5;13(2):e0192498. doi: 10.1371/journal.pone.0192498

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© 2018 Tang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — PNU and MLA changed only the membrane expression of NMDAR-containing NR2B subunit. Western blot for the expression of α7nAChR and the membrane, intracellular and total expression of NMDAR subunits was performed on 2-month-old rat hippocampus neurons (A, C, D and E). Epidermal growth factor receptor (EGFR) and β-actin were used as the loading controls. Pooled densitometric results for α7nAChR, NR1, NR2A and NR2B, with the band intensity of control group assigned the value of 1. Sevoflurane induced significant decreases of the expression level of α7nAChR. n = 8 for each group; compared with group C, *P<0.01, ANOVA. PNU-282987 prevented a change in the α7nAChR expression. n = 8 for each group; compared with group S, ^#P<0.01, ANOVA. MLA significantly decreased the expression level of α7nAChR. n = 8 for each group; compared with group C, *P<0.01, ANOVA (B). Sevoflurane induced significant decreases in the membrane expression level of NMDAR subunits (NR1, NR2A and NR2B). n = 8 for each group; compared with group C, *P<0.01, ANOVA. PNU-282987 prevented a change in the membrane NR2B expression but not in that of NR1 or NR2A. n = 8 for each group; compared with group S, ^#P<0.01, ANOVA. MLA significantly decreased the expression level of membrane NR2B but had no effect on the membrane protein levels of NR1 and NR2A. n = 8 for each group; compared with group C, *P<0.01, ANOVA (F). Sevoflurane induced significant increases in the intracellular expression level of NMDAR subunits (NR1, NR2A and NR2B). n = 8 for each group; compared with group C, *P<0.01, ANOVA. PNU-282987 prevented a change in the expression of intracellular NR2B, but not NR1 and NR2A. n = 8 for each group; compared with group S, ^#P<0.01, ANOVA. MLA significantly increased the expression level of intracellular NR2B but had no effect on the intracellular protein levels of NR1 and NR2A. n = 8 for each group; compared with group C, *P<0.01, ANOVA (G). The total expression levels of NR1, NR2A and NR2B were not different among the groups (H).