Skip to main content
. 2018 Feb 5;13(2):e0192498. doi: 10.1371/journal.pone.0192498

Fig 4. In vitro, sevoflurane decreased the expression of SFK and membrane NMDAR subunits.

Fig 4

PNU increased the expression level of SFK and the membrane NR2B subunit, and MLA had opposite effects. Western blot for the expression of SFK as well as the membrane, intracellular and total expression of NMDAR subunits was performed on rat hippocampus neurons of 7-day-old rat brain slices (A, C, D and E). Epidermal growth factor receptor (EGFR) and β-actin were used as the loading controls. Pooled densitometric results for SFK, NR1, NR2A and NR2B, with the band intensity of control group assigned the value of 1. Sevoflurane induced significant decreases of the expression level of SFK. PNU-282987 increased SFK expression. MLA significantly decreased the expression level of SFK. n = 8 for each group; compared with group C, *P < 0.01, ANOVA (B). Sevoflurane induced significant decreases in the membrane expression level of NMDAR subunits (NR1, NR2A and NR2B). PNU-282987 increased the membrane NR2B expression but had no effect on the membrane protein levels of NR1 and NR2A. MLA significantly decreased the expression level of membrane NR2B but not of NR1 or NR2A. n = 8 for each group; compared with group C, *P < 0.01, ANOVA (F). Sevoflurane induced significant increases in the intracellular expression level of NMDAR subunits (NR1, NR2A and NR2B). PNU-282987 decreased the intracellular NR2B expression but had no effect on membrane protein level of NR1 and NR2A. MLA significantly increased the expression level of intracellular NR2B but not of NR1 or NR2A. n = 8 for each group; compared with group C, *P < 0.01, ANOVA (G). The total expression levels of NR1, NR2A and NR2B were not different among the groups (H).