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. 2018 Feb 5;13(2):e0192498. doi: 10.1371/journal.pone.0192498

Fig 5. SFK inhibitor inhibited the upregulation of NMDAR-containing NR2B subunit induced by PNU.

Fig 5

Western blot for the expression of the membrane, intracellular and total expression of NR2B was performed on rat hippocampus neurons from 7-day-old rat brain slices (A, B and C). Epidermal growth factor receptor (EGFR) and β-actin were used as the loading controls. Pooled densitometric results for NR2B, with the band intensity of control group assigned the value of 1. PNU-282987 significantly increased the membrane NR2B expression. n = 8 for each group; compared with group C, *P < 0.01, ANOVA. SFK inhibitor PP2 prevented changes in membrane NR2B expression. n = 8 for each group; compared with group P, #P < 0.01, ANOVA (D). PNU-282987 significantly decreased the intracellular NR2B expression. n = 8 for each group; compared with group C, *P < 0.01, ANOVA. SFK inhibitor PP2 prevented changes in intracellular NR2B expression. n = 8 for each group; compared with group P, #P < 0.01, ANOVA (D). The total expression levels of NR2B were not different among the groups (D).