Figure 2. Human serum IgM (left) and IgG (right) binding to aortic endothelial cells (AECs).
AECs were cultured from a wild-type (WT) pig, an α1,3-galactosyltransferase gene-knockout (GTKO) pig expressing the human complement-regulatory protein, CD46, and a GTKO/CD46 pig that did not express NeuGc (GTKO/CD46/CMAHKO). Human IgM and IgG binding to GTKO/CD46/CMAHKO pig cells is almost at the level of binding to human aortic endothelial cells. (* = p<0.05) (Modified from Lee W, et al. Xenotransplantation 2016;23:137–150)