Table 2. Plasmids used in this study.
| Plasmid # | Plasmid name | Description | Bacterial selection | Reference |
|---|---|---|---|---|
| 8 | pRS316 | pRS316, cen, Ura3 | Amp | Sikorski and Hieter, 1989 |
| 10 | HA-Fzo1 on pRS316 | HA-Fzo1 on pRS316, Fzo1 prom, cen, Ura3 | Amp | Anton et al. (2013) |
| 14 | HA-Fzo1-K464R on pRS316 | HA-Fzo1K464R on pRS316, Fzo1 prom, cen, Ura3 | Amp | Anton et al. (2013) |
| 58 | YEplac181 | YEplac181, 2µ, Leu2 | Amp | Gietz and Sugino, 1988 |
| 59 | Ubp2-Flag on YEplac181 | Ubp2-Flag on YEplac181, Adh1 prom, 2µ, Leu2 | Amp | Anton et al. (2013) |
| 60 | Ubp2-C745S-Flag on YEplac181 | Ubp2C745S-Flag on YEplac181, Adh1 prom, 2µ, Leu2 | Amp | Anton et al. (2013) |
| 61 | Ubp12-Flag on YEplac181 | Ubp2-Flag on YEplac181, Adh1 prom, 2µ, Leu2 | Amp | Anton et al. (2013) |
| 62 | Ubp12-C372S-Flag on YEplac181 |
Ubp2C372S-Flag on YEplac181, Adh1 prom, 2µ, Leu2 | Amp | Anton et al. (2013) |
| 63 | YEplac195 | YEplac195, 2µ, Ura3 | Amp | Gietz and Sugino, 1988 |
| 65 | Ubp12C372S on YEplac195 | Ubp12C372S (non-tagged) on YEplac195, 2µ, Ura3 | Amp | this study |
| 70 | mt-GFP on pYX142 | mt-GFP on pYX142, cen, Leu2 | Amp | Westermann and Neupert, 2000 |
| 75 | Cdc48 wt on pRS313 | Cdc48 wt on pRS313, cen, His3 | Amp | Esaki and Ogura (2012) |
| 79 | pRS313 | pRS313, cen, His3 | Amp | Sikorski and Hieter, 1989 |
| 150 | Cdc48-A547T on pRS313 | Cdc48A547T on pRS313, cen, His3 | Amp | this study |
| 341 | Ub on pKT10 | Ub on pK10, 2µ, Ura3 | Amp | Tanaka et al., 1990 |
| 342 | Ub-K48R on pKT10 | UbK48R on pK10, 2µ, Ura3 | Amp | Tanaka et al., 1990 |
| 343 | Ub-K63R on pKT10 | UbK63R on pK10, 2µ, Ura3 | Amp | Tanaka et al., 1990 |
| 344 | Ub-K48R,K63R on pKT10 | UbK48R,K63R on pK10, 2µ, Ura3 | Amp | Tanaka et al., 1990 |
| 356 | Myc-Ub on pRS426 | pCup1-Myc-Ub on pRS426, 2µ, Ura3 | Amp | Li et al., 2015 |
| 375 | pRS426 | pRS426, 2µ, Ura3 | Amp | Li et al., 2015 |