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. Author manuscript; available in PMC: 2018 Feb 5.
Published in final edited form as: J Mol Biol. 2016 Nov 16;429(4):562–573. doi: 10.1016/j.jmb.2016.11.008

Fig. 5.

Fig. 5

Mutational analysis of RasIn1 reveals functionally important residues. (a) In vitro pull-down efficiency of RasIn1 point mutants. Radiolabeled point mutants of RasIn1 were tested for binding to immobilized K-Ras-GTP. The percentage of radioactive counts bound to beads is shown. S24, R72, and R77 are critical positions for binding. (b) BC and FG loop mutations observed in the top 20 single point mutants of RasIn1 in high-throughput sequencing of pool 6 (Fig. S2). Residue numbers are shown above the sequence.