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. Author manuscript; available in PMC: 2018 Aug 5.
Published in final edited form as: Dev Neurosci. 2017 Aug 5;39(6):443–459. doi: 10.1159/000477898

Fig. 6.

Fig. 6

rhIGF-1 attenuated neonatal LPS-induced reduction in the number of TH+ cells in the locus coeruleus (LC) of the P21 rat brain. Representative photomicrographs of TH+ neurons in the VTA region for the Saline+Vehicle group (A), the LPS+Vehicle group (B), and the LPS+IGF-1 group (C). The corresponding intensity profiles of TH expression were presented under the photomicrographs of TH+ cells in the LC region (D-F). LPS injection resulted in a loss of TH+ staining (B&E). rhIGF-1 attenuated LPS-induced loss of TH+ staining (C&F). Quantification of TH+ cells and NeuN+ cells in the LC are shown in G and H, respectively. Quantification of the TH+ cells in the SN, VTA, OB and LC are shown in I. The results are expressed as the mean+SEM of six animals in each group and analyzed by two-way ANOVA, followed by the Student-Newman-Keuls test. *P<0.05 represents significant difference for the LPS+Vehicle group compared with the Saline+Vehicle group. #P<0.05 represents significant difference for the LPS+IGF-1 group compared with the LPS+Vehicle group. &P<0.05 represents significant difference for the LPS+Vehicle group in VTA or OB regions compared with the LPS+Vehicle group in the SN.