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. Author manuscript; available in PMC: 2018 Feb 5.
Published in final edited form as: Shock. 2009 May;31(5):466–472. doi: 10.1097/SHK.0b013e31818ec47d

FIG. 4. Hypertonic saline alters IκBα phosphorylation and degradation dynamics.

FIG. 4

A, Western blot of whole cell lysate does not find phosphorylated IκBα in unstimulated cells. TNF-α provokes a marked increase in phosphorylated IκBα at 5 min that degrades by 10 min. Hypertonic saline significantly decreases the amount of TNF-α–induced phosphorylation that degrades by 10 min as in controls. Hypertonic saline does not change total IκBα concentration in unstimulated cells. TNF-α stimulates IκBα degradation at 5 min that is complete by 10 min. Hypertonic saline inhibits degradation with a significant amount remaining at 10 min and a small but detectable amount remaining at 30 min. B, Hypertonic saline significantly decreases the amount of phosphorylated IκBα at 5 min of TNF-α stimulation. Densitometry data from four separate experiments are presented as SEM (*P < 0.001). C, TNF-α stimulates near complete total IκBα degradation at 10 min. Hypertonic saline delays the same level of degradation to approximately 30 minutes. Hypertonic saline significantly decreases total IκBα degradation at 10 min (*P < 0.001). Densitometry data of four separate experiments are presented as SEM.