Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 5;8:2431. doi: 10.1038/s41598-018-20859-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Overexpression of miR-203a suppressed HCCs metastasis, invasion and angiogenesis. (A) Transwell analysis of SMMC-7721 and Hep3B cells after transfected with miR-203a and its ctrl, quantitative analysis of the invasion rates by solubilization of crystal violet and spectrophotometric reading at OD 490 nm. (B) Wound-healing assays of SMMC-7721 cells and Hep3B after treatment with miR-ctrl, miR-203a. The relative wound closure (100%) represents the metastasis capacity of SMMC-7721 and Hep3B cells. (C Left) Tube formation assay in HUVECs after transfected with miR-ctrl and miR-203a in vitro. (C Right) Angiogenesis assay in SMMC-7721 after transfected with miR-203a and its ctrl. (D) Effects of miR-203a overexpression on protein expression of EMT markers and related proteins by western blot. β-actin was detected as an internal control (*P < 0.05; **P < 0.01, Student’s t test).