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. 2018 Feb 5;8:2431. doi: 10.1038/s41598-018-20859-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-203a inhibits cells invasion and angiogenesis through HOXD3. (A) The invasion viability of SMMC-7721 cells and Hep3B was determined using the Transwell invasion assay, quantitative analysis of the invasion rates by solubilization of crystal violet and spectrophotometric reading at OD 490 nm. (B) Wound-healing assays of HCCs after treatment with si-ctrl and si-HOXD3. Representative images were captured at 0 and 48 h after transfection of si-ctrl and si-HOXD3. (C) Angiogenesis assay in HUVECs transfected with si-HOXD3 and its control. (D) The expression of VEGFR and downstream genes E-cadherin N-cadherin, MMP9 and by using western blot. β-actin as an internal control was detect (**P < 0.01, Student’s t test).