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. 2018 Feb 5;8:2431. doi: 10.1038/s41598-018-20859-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-203a rescues HOXD3 induced cellular invasion and angiogenesis in HCCs. (A) SMMC-7721 and Hep3Bcells invasion was verified by Transwell assay. Quantitative analysis of the invasion rates by solubilization of crystal violet and spectrophotometric reading at OD 490 nm. (B) The wound-healing assay was performed to determine the immetastasis of HCCs. (C) Tube formation assay in HUVECs transfected with ASO-NC and ASO-miR-203a. (D) The expression of VEGFR, EMT marker genes E-cadherin, N-cadherin and MMP9 were assayed by western blot analysis. β-actin was also detected as an internal control (*P < 0.05; **P < 0.01, Student’s t test).