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. 2018 Feb 5;8:2425. doi: 10.1038/s41598-018-20710-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SS-hAFSCs and RS-hAFSCs differ for the expression of CD105 and CD90. (a) Isolation and representative inverted light microscopy images of human mid-trimester round-shaped (RS-hAFSCs) and spindle-shaped (SS-hAFSCs) amniotic fluid stem cells. (b) Example confocal immunostaining images demonstrating cytoplasmic CD117 staining (in green) in SS-hAFSCs and RS-hAFSCs counterstained with nuclear DAPI (blue). (c) Flow cytometry showing expression of CD73 and absence of expression of CD34 and CD45 in SS-hAFSCs and RS-hAFSCs, with only SS-hAFSCs expressing the cell surface markers CD90 and CD105. The red tracing shows the isotype control and the blue tracing shows the primary antibody.