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. 2018 Feb 5;8:2337. doi: 10.1038/s41598-018-19421-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mitophagy and autophagy analyses by confocal microscopy. (a) Primary human IMR90 cells cultivated under OR, GR and OGR for 24 h and 4 h prior to the harvesting cells were treated with and without BafA. Afterwards cells were fixed and immunostained with BNIP3L/Nix and LC3 antibodies and analyzed microscopically. Representative photographs of cells are shown.