Table 1.

Inhibitory effects of inhibitors on NADH-ubiquinone-1 oxidoreductase activity of the purified Ndi1.

Compound	IC50a (nM)	Inhibition modeb	Kic (nM)	αc (α > 0)
Stigmatellin	107 ± 21.0	competitive	93.9 ± 30.4	~6.33×1020
AC0-12	115 ± 18.0	mixed	14.0 ± 1.9	13.0±11.7
Myxothiazol	308 ± 22.9	mixed	48.8 ± 5.2	70.2±192
Ascochlorin	955 ± 139	mixed	728 ± 191	2.64±1.87

^aEach value represents the mean ± S.E.M of three independent experiments.

^bInhibition modes for ubiquinone-1 were determined by the intersection point in Lineweaver-Burk plots (Fig. 2B–E).

^cThe values for the inhibition constant K_i and α were determined by fitting the experimental data, which are the same as the Lineweaver-Burk plots, to non-linear regression of mixed type inhibition using the GraphPad Prism 6 software program. Each value represents the mean ± S.D.