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. 2018 Feb 5;8:2335. doi: 10.1038/s41598-018-20597-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Release of IDE and LDH from HEK-293 cells. HEK-293 cells were cultured in DMEM media with 10% FBS. At the time periods indicated aliquots were taken and used for Western blot analysis for IDE (■) or LDH (◻). The % released is based on the Western blot signal in the media relative to the total Western blot signal (media plus cell lysate). Indicated points are the mean of results from five independent experiments with the error bars represent +/− the standard deviation of the measurements. The lines show linear regression fits to the IDE values (solid line) or LDH values (dashed line).