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. 2018 Feb 5;8:2382. doi: 10.1038/s41598-018-20829-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation and characterization of the Sema3a-CreERT2 knock-in mouse line. (a) Schematic diagrams showing the wild-type (wt) allele and the CreERT2 knock-in Sema3a allele. The arrows indicate the primers that were designed for genotyping. (b) Genotyping of the Sema3a-CreERT2 allele by PCR. The band size is presented on the left. M, molecular marker. (c) Immunostaining for oestrogen receptor (ESR) as a surrogate for Sema3a on E12.5, E14.5 and E19.5 Sema3a-CreERT2 heart sections. The arrows indicate Sema3a⁺ cardiomyocytes. Scale bars, 100 µm.