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. 2018 Feb 5;8:2382. doi: 10.1038/s41598-018-20829-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of the numbers of Sema3a⁺ cardiomyocytes in the adult heart. (a) Immunostaining for RFP and TNNI3 on adult heart sections. IVS, interventricular septum; RVW, right ventricular wall. Scale bars: white, 100 µm; yellow, 50 µm. (b) Image of isolated cardiomyocytes from the adult Sema3a CreERT2; R26-tdtomato heart showed Sema3a⁺ cardiomyocytes. (c) Quantification of the percentages of RFP⁺ cardiomyocytes in the heart sections. (d) Quantification of the percentages of RFP⁺ cells from among isolated cardiomyocytes. Each image is representative of 5 individual samples.