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Histograms of platelet size distribution, including genuine platelets and platelet-like particles. Platelet-rich plasma (PRP) fraction after centrifugation with glass beads, without added CaCl2 (A). PRP-FM after digestion with tissue-plasminogen activator (t-PA) for 3 h (B), 6 h (C), or 24 h (D). As comparison, PRP-FM after 5 h digestion with trypsin (E) or trypsin + EDTA (F). Abbreviations: PDW, platelet distribution width; MPV, mean platelet volume; P-LCR, platelet–large cell ratio.
