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. 2018 Feb 5;8:2370. doi: 10.1038/s41598-018-20813-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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eGFP expression and specificity during early rod production in NRL^+/eGFP OVs. To examine early rod production in NRL^+/eGFP OVs, OVs at d90 of differentiation were analyzed by immunocytochemistry. (A–C). All NRL-eGFP+ cells co-expressed CRX (arrowheads), but not all CRX+ cells expressed eGFP. (D–I) NRL-eGFP (D) co-labeled with NRL (E) and NR2E3 (F), demonstrating specific expression of eGFP in rod photoreceptors (merged images in (G–I). Examples of co-localization of NRL and NR2E3 with eGFP highlighted by arrowheads in panels D–I. Scale bars = 50 µm.