FIGURE 1.

Phenotypical characterization of proliferating SHSY5Y cells (SHSY5Ywt). (A) Confocal microscopic images showing the intracellular distribution of the neuronal markers Nestin, Tubulin-β3 and NeuN, of the DA neuron specific marker TH, and of the most important mediator of the canonical Wnt signaling β-catenin. For Tubulin-β3, Nestin, TH, and β-catenin immunostaining, cell nuclei were counterstained with propidium iodide (PI, red hue); for NeuN, cell morphology was visualized by differential interference contrast (DIC). For each marker, both single staining and merged images with PI or DIC are shown. Bars: 25 μm. (B) Western blot analysis of expression levels of Nestin, Tubulin-β3, NeuN, TH, Fzd1 receptor and β-catenin in total cell lysates from SHSY5Ywt cells.