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. 2018 Feb 1;10:20. doi: 10.3389/fnagi.2018.00020

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Copyright © 2018 Colini Baldeschi, Pittaluga, Andreola, Rossi, Cozzolino, Nicotera, Sferrazza, Pierimarchi and Serafino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Atrial natriuretic peptide pre-treatment prevents 6-OHDA toxicity on SHSY5Ywt and SHSY5Ydiff cells. (A,B) Phase contrast microscopy of SHSY5Ywt (A) and SHSY5Ydiff (B) cells exposed to 50 and 100 μM of 6-OHDA, respectively, for mimicking the neurodegeneration of PD. To verify the ability of ANP in preventing the 6-OHDA induced cytotoxicity, cells were pre-treated with 100 nM of the natriuretic peptide, 30 min or 24 h prior to 6-OHDA addition to cell culture medium. Original magnification: left panels: 10x; right panels: 20x. (C,D) Cell viability assay performed on SHSY5Ywt (C) and SHSY5Ydiff (D) cells after 24 h of 6-OHDA treatment by Trypan blue dye exclusion method; results, reported as percentage of live/dead cells, are mean of three independent experiments. ^∗Significance vs untreated control (Ctr); ^#Significance vs 6OH-Dopa: ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; the mean ± SD; n = 3.