Table 3.
Resuming scheme of neuroprotective effect of ANP pre-treatment on SHSY5Ywt and SHSY5Ydiff cells subjected to 6OH-Dopa insult.
| Parameter evaluated | Related function | ANP 100 nM | 6OH-Dopa 24 h | Pre-ANP 30 min + 6OH-Dopa | Pre-ANP 24h + 6OH-Dopa |
|---|---|---|---|---|---|
| Cell adhesion | Cell integrity | Unmodified | Reduced of ∼50% | Preserved | Preserved |
| Neuritic network | Cell integrity and functionality | Unmodified | Destroyed | Partially preserved | Partially preserved |
| Cell viability | Cell survival | Unmodified | Reduced of 50–60% | Significantly recovered (p < 0.01) | Significantly recovered (p < 0.01) |
| β-catenin (protein) |
Canonical Wnt signaling mediator | Up-regulated (β-cat stabilization) |
Down-regulated | Significantly restored near control values (p < 0.001) | Significantly restored near control values (p < 0.001) |
| pβ-catenin | Preliminary to β-cat degradation | Down-regulated (decreased β-cat degradation) | Up-regulated (increased β-cat degradation) | Significantly restored near control values (p < 0.001) | Significantly restored near control values (p < 0.001) |
| pAKTThr308 (active form) |
Survival factor involved in neuroprotection | Up-regulated | Down-regulated in wt Up-regulated in diff | Up-regulated | Up-regulated |
| DJ1 | Survival factor involved in neuroprotection, dysfunctional or low expressed in PD | Up-regulated in wt Unmodified in diff | Down-regulated | Restored near control values | Restored near control values |
| Nurr1 | DA neuron specific marker; expression regulated by nuclear β-cat | Up-regulated | Down-regulated | Significantly restored near control values (p < 0.01) | Significantly restored near control values (p < 0.05) |
| Tyrosine hydroxylase | DA neuron specific marker; expression regulated by Nurr-1 | Up-regulated | Down-regulated | Restored near control values | Restored near control values |