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. 2018 Feb 1;9:105. doi: 10.3389/fmicb.2018.00105

FIGURE 4.

FIGURE 4

Comparisons of the proteolytic activity (A) and ComX biological activity decay (B) of different B. subtilis 48 h floating biofilm spent media. (A) Proteolytic activity of the spent medium from floating biofilms. Different B. subtilis PS-216 strains (wt, degQ:tet, comP::cat) were grown for 48 h at 37°C in MSgg medium. Where indicated, subtilisin or EDTA was added to spent media after harvest in order to increase or inhibit the proteolytic activity of the media, respectively. Averages and SEM of three independent biological replicates are shown. A Mann–Whitney U-test was performed to determine statistical significance of discussed measurements (∗p < 0.05). (B) The ComX biological activity decay of the same harvested floating biofilm spent medium as above using a signal deficient biosensor strain BM1456. Averages and SEM of three independent biological replicates are shown. A Mann–Whitney U-test was performed to determine statistical significance of discussed measurements (∗p < 0.05).