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UPEC strain UTI89 colonization on fixed HTB9 uroepithelial cell layers under flow of artificial urine medium. Four individual flow chamber runs are shown together to visualize the high degree of reproducibility from experiment to experiment. Eleven 1,100 × 850 μm sites were monitored in each flow chamber over 24 h. Error bars indicate ±1 standard deviation. ROI, region of interest.
