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. 2018 Feb 1;8:16. doi: 10.3389/fcimb.2018.00016

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Copyright © 2018 Pedersen, Grønnemose, Stærk, Asferg, Andersen, Kolmos, Møller-Jensen and Andersen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bacterial seeding efficiency. Graphs showing the mean, total number of microcolonies (events) detected at 1,100 × 850 μm sub-locations at each 20-min time point during infection experiments with three different pathogens. Data for each type of experiment were pooled from all conducted flow chamber experiments. Peaks indicate the maximum number of bacteria which succeed in establishing a microcolony after initial seeding. (A) UPEC strains UTI89wt and UTI89ΔfimH grown on uroepithelial cell layers in a flow of artificial urine (AU). UTI89wt was precultured in either AU or LB prior to the experiment, as indicated. (B) STEC growth on intestinal epithelial cells (T84). (C) S. aureus colonization on endothelial cell layers (EA.hy926).