Table 1.

Sequence variations of plasmid pME6031 confirmed by Next Generation Sequencing.

Location	Observation	Comment
1	Deletion of G	Removes a BamHI site
3802	Deletion of G	Between pVS1 ori and p15A ori
5137	Deletion of C	Near T4 transcription terminator
5526(CAGC)5527	Insertion of CAGC	Adjacent to the MCS
6079(del)6146	Deletion	66 base deletion; GC-rich region
8286(GG)8287	Insertion of GG	Adjacent to XbaI site

Numbering is according to Accession number AF118811.