Table 3.
List of new vectors created from pME6031.
| Plasmid name | Relevant characteristics |
|---|---|
| pSW001 | pME6031 with high copy number mutation |
| pSW002 | pME6031 with restriction sites1 removed |
| pSW003 | pME6031 with high copy number mutation2 + restriction sites1 removed |
| pSW002-Pc | Empty vector + Pc promoter |
| pSW002-Pc-mTurquoise2 | Pc promoter + cytosolic expression of mTurquoise2 |
| pSW002-Pc-mNeonGreen | Pc promoter + cytosolic expression of mNeonGreen |
| pSW002-Pc-mRuby2 | Pc promoter + cytosolic expression of mRuby2 |
| pSW002-Pc-DsRed-Express2 | Pc promoter + cytosolic expression of DsRed-Express2 |
| pSW002-Pc-E2-Crimson | Pc promoter + cytosolic expression of E2-Crimson |
| pSW002-Pc-TorA | Empty vector + Pc promoter + TorA signal peptide |
| pSW002-Pc-TorA-mTurquoise2 | Pc promoter + periplasmic targeting of mTurquoise2 |
| pSW002-Pc-TorA-mNeonGreen | Pc promoter + periplasmic targeting of mNeonGreen |
| pSW002-Pc-TorT | Empty vector + Pc promoter + TorT signal peptide |
| pSW002-Pc-TorT-mTurquoise2 | Pc promoter + periplasmic targeting of mTurquoise2 |
| pSW002-Pc-TorT-mNeonGreen | Pc promoter + periplasmic targeting of mNeonGreen |
| pSW002-PpsbA | Empty vector + psbA promoter |
| pSW002-PpsbA-mTurquoise2 | psbA promoter + cytosolic expression of mTurquoise2 |
| pSW002-PpsbA-mNeonGreen | psbA promoter + cytosolic expression of mNeonGreen |
| pSW002-PpsbA-DsRed-Express2 | psbA promoter + cytosolic expression of DsRed-Express2 |
| pSW002-PpsbA-E2-Crimson | psbA promoter + cytosolic expression of E2-Crimson |
| pSW003-Pc-mNeonGreen | High copy vector + Pc promoter + cytosolic expression of mNeonGreen |
| pSW003-PpsbA-mNeonGreen | High copy vector + psbA promoter + cytosolic expression of mNeonGreen |
The format of each plasmid name is: base plasmid-promoter-periplasmic signal sequence (if present)-fluorescent protein. 1NotI(844), NotI(2377), NotI(3667), XbaI(8291) and BamHI(1). 2RepA(A246V).