Table 4.

Properties of fluorescent proteins used in this study.

Protein	λex (nm)	λem (nm)	𝜀 (mM-1 cm-1)a	ϕb	Brightnessc	Reference
mTurquoise2	434	474	30	0.93	27.9	Goedhart et al., 2012
mNeonGreen	506	517	116	0.80	92.8	Shaner et al., 2013
mRuby2	559	600	113	0.38	43	Lam et al., 2012
DsRed-Express2	554	591	35.6	0.42	15.0	Strack et al., 2008
E2-Crimson	611	646	126	0.23	29	Strack et al., 2009

^aExtinction coefficient. ^bQuantum yield. ^cBrightness is the product of the extinction coefficient and quantum yield.