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. 2018 Feb 1;12:22. doi: 10.3389/fncel.2018.00022

FIGURE 2.

FIGURE 2

Analysis of synaptic proteins in organotypic hippocampal slices treated with SPARC reveals an increase in GluA1 levels and GluA1-containing AMPAR complexes. (A) Representative immunoblots showing total (left panel, ‘crude’) and synaptic (right panel) proteins (GluA1, GluA2, GluN1, and PSD-95) from synaptosome preparations of control and SPARC-treated (0.5 μg/ml, 48 h) organotypic hippocampal slices. (B) Hippocampal slices treated with SPARC have increased total and synaptic GluA1 [2-tailed, 1-sample t-test; p = 0.004, n = 4)] relative to control, but no significant change in GluA2 levels (n = 6). (C) SPARC-treated hippocampal slices show an increase in GluA1–GluA1 AMPAR complexes, whereas we did not observe a change in GluA1–GluA2 containing complexes [2-tailed, 1-sample t-test; p = 0.024, n = 4)].