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. 2018 Feb 5;8:2410. doi: 10.1038/s41598-018-20000-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of MDM2 inhibitors on ability of senescent cells to stimulate cancer cell aggressiveness. (A,B) ZR75-1 human breast cancer cells were cultured in the presence of CM from DMSO- or MI-63-treated (mock or IR) HCA2 fibroblasts for 3 d, and immunostained for ZO-1, cytokeratin 18 or vimentin. (A) Representative images of the staining. (B) Quantification of staining (percentage of positive cells). (C) CM described in (A) was assayed for ability to stimulate ZR75-1 cancer cells to invade a basement membrane. Invasion stimulated by CM from DMSO-treated mock fibroblasts was given a value of one, and other conditions were normalized to this value. Data are means of 4 independent experiments (*p < 0.05, t-test). Error bars indicate standard deviation of the mean.