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. 2018 Feb 5;8:2346. doi: 10.1038/s41598-018-20703-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Diastolic dysfunction is evident in diabetic female but not male mice. (A) Representative echocardiography traces from pulse-wave (blood flow) Doppler imaging. (B) Representative echocardiography traces from A4C view of mitral valve tissue Doppler. (C) Ratio of flow Doppler E wave amplitude to tissue Doppler E′ wave amplitude. (D) Ratio of flow Doppler E wave to A wave amplitude. (E) Mitral valve flow Doppler deceleration time. (F) Ratio of tissue Doppler E′ wave to A′ wave amplitude. Data are presented as mean ± SEM. n = 5/group. *p < 0.05, 2-way ANOVA, annotated with LSD post hoc analyses.