Fig. 2.

Nanoformulation stability and release kinetics. Nanoformulations were synthesized by high-pressure homogenization using poloxamer 407 (P407) as the excipient for DTG and MDTG. a–c Formulation stability (up to 134 days) was measured by (a) particle hydrodynamic diameter (size), (b) polydispersity index (PDI), and (c) zeta potential as determined by dynamic light scattering (DLS). NDTG and NMDTG stability were tested at both 4 °C and 25 °C. d, e Nanoparticle release kinetics were assessed by drug released from the nanoparticles during storage, in (d) freshly manufactured (neat) formulation and (e) formulation diluted for in vivo administration. All results are shown as the mean ± SEM of at least three replicates