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. 2018 Feb 5;8:2367. doi: 10.1038/s41598-018-20919-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OASS activity and OASS content in soybean nodules. Protein extracts obtained from 20 day old nodules of nontransgenic and transgenic plants were assayed for OASS activity (A). Average values are shown ± SD (n = 3). Total nodule proteins were separated on 15% SDS-PAGE gels and either stained with Coomassie Blue (B) or transferred to nitrocellulose membrane and probed with antiserum against soybean OASS (C). Antibody reactivity was detected by using anti-rabbit IgG-horseradish peroxidase conjugate followed by chemiluminescent detection. Lane 1 nodule protein from non-transformed wild-type, lanes 2 to 4 nodule protein from OASS transformed transgenic plants CS02, CS022, and CS027, respectively. Autoradiographs were scanned with an Epson Perfection V700 PHOTO, with image acquisition done through Adobe Photoshop, and scanned at 300 dpi using 24-bit color picture setting. Images were processed and cropped with PowerPoint.