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. 2018 Feb 5;8:2367. doi: 10.1038/s41598-018-20919-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Anatomy of soybean nodules. Nodules collected at 15 days after infection were thick-sectioned and viewed under light microscope (A,B). For ultrastructral observation thin-sections were examined by electron microscopy (C,D). Note the cells from non-transformed wild-type nodules are filled with bacteria (A,C) while the cells from OASS overexpressing transgenic nodule are not completely filled by bacteria (B,D). Also note the presence of prominent starch grains in the cells of from OASS overexpressing transgenic nodule (D). B, bacteroid; IC, infected cells; N, nucleus; PHB, polyhydroxybutyrate; S, starch; UIC, uninfected cell; V, vacuole.