Fig. 5.
Effect of the method of RNA isolation on the performance (a) and sensitivity (b) of detection of PVY by Y4 RT-LAMP. (a) Amplification plots recorded for RNA purified from plants infected with PVY by magnetic capture (i) according to the manufacturer (ii) by a modified magnetic procedure and (iii) by a shortened magnetic procedure. (b) Comparison of the effect of the different RNA isolation methods on sensitivity. Sap from plants infected with PVY was serially diluted up to 2 × 106 times, and total RNA was purified from each dilution by the magnetic capture procedure recommended by the manufacturer, by the shortened magnetic capture procedure, and by silica capture. Time-to-positive results are shown as a function of the logarithm of the dilution factor. The data are averages from at least two independent experiments performed in triplicate. Error bars indicate standard deviation