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. 2018 Feb 5;8:2351. doi: 10.1038/s41598-018-20731-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Interdependent membrane expression of cubilin and amnionless. (a) Rat cubilin (full-length and mini cubilin; 1–930) and human amnionless (full length) constructs encoded by plasmid cDNA used for transient transfection of cultured cells. (b,d,f) Non-permeabilised HEK293T, MDCK cells and RPTECs transfected with the indicated vectors were fixed and stained for membrane-targeted cubilin (red). GFP-tagged amnionless is shown in green and DAPI nuclear staining in blue. (Scale bar: 10 µm). (c,e,g) Plasma membrane expressions of cubilin were obtained in permeabilised HEK293T, MDCK cells and RPTECs cotransfected with amnionless. Pictures are confocal sections taken from the middle height of cells (top) and X-Z vertical sections (bottom) (Scale bar: 10 µm).