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. 2018 Feb 5;8:2351. doi: 10.1038/s41598-018-20731-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cubilin mutation inhibits its trafficking from endoplasmic reticulum to Golgi apparatus. (a and b) HEK293T cells transfected with indicated vector were fixed, permeabilised and labeled for calnexin (a), the endoplasmic reticulum marker, or golgin 97 (b), a marker of the Golgi apparatus, followed by Alexa Fluor 555-conjugated antibody (red). GFP-tagged cubilin is shown in green and DAPI nuclear staining in blue. Pictures are confocal sections taken from the middle height of cells. (Scale bar: 10 µm).