Fig. 5.

BMI1-specific antagonist inhibits AR-signaling pathway. C4-2 (a) or 22Rv1 (b) was treated with PTC209 in indicated concentration for 48 h, BMI1 and AR were tested by western blot, and GAPDH served as loading control. c C4-2 cells were treated for 48 h as labeled, vehicle as control, and the concentration of each drug was PTC209 5 µM, MG132 20 µM, NH₄Cl 10 mM, and chloroquine 200 µM. Total cell lysates were blotted for AR and BMI1, while GAPDH served as loading control. d C4-2 cells were treated with PTC209 (5 µM), and after 24 h, cells were infected with BMI1 lentivirus, BMI1-RING lentivirus, or BMI1ΔRING lentivirus as indicated in the presence of PTC209, and cells were lysed after another 24 h and probed by indicated antibodies. All experiments were biologically repeated at least three times. Representative images are shown