Fig. 5.

The effect of the nrsym1 mutation or NRSYM1 constitutive expression on gene expression. a–d Real-time RT-PCR analysis of (a) CLE-RS2, (b) LjNIA, (c) LjNIR1, and (d) CLE-RS1 expression in roots of WT and the nrsym1-1 mutants. e–h Real-time RT-PCR analysis of (e) CLE-RS2, (f) LjNIA, (g) LjNIR1, and (h) CLE-RS1 expression in transgenic hairy roots produced from WT containing the pLjUBQ:GUS or pLjUBQ:NRSYM1 constructs. Each cDNA sample was prepared from total RNA derived from an uninoculated whorl of roots grown in the presence of 0 or 10 mM KNO₃ for 24 h. The expression of LjUBQ was used as the reference. Error bars indicate SEM (n = 3 independent pools of roots). *P < 0.05 by Student’s t-test. ns, not significant. Degrees of freedom are shown above the t-values