Fig. 2. Results of phylogenetic analysis of Japanese encephalitis virus strains isolated from Chinese swine herds based on assessment of the capsid/premembrane protein (C/PrM) nucleotide sequences. The multiple sequence alignments were obtained by using MEGA software (ver. 5.0; Molecular Evolutionary Genetics Analysis). The tree was constructed by applying the neighbor-joining method. Scale bar indicates number of nucleotide substitutions per site. Bootstrap confidence limits are shown at each node. The Dengue 1 virus (strain GZ2002) was used as an outlier group. Those strains (marked by ▲ or ●) were isolated and identified from pigs between 2006 and 2012 in China.