Figure 2.

Mouse and human prostate tumors express CAR and are efficiently infected by Ad-mCherry. (a) A representative area of a human prostate tumor microarray (RPCI) stained with anti-CAR antibodies (T—tumor and N—normal prostate tissue samples). (b) Expression of CAR (green) in TRAMP-C2 cells revealed by immunofluorescent staining with anti-CAR antibodies. (c) A TRAMP mouse prostate tumor was injected with Ad-mCherry (5 × 10⁸ v.p./tumor). Twenty-four hours later, CAR (green) and mCherry (red) expression were detected in tumor epithelial cells positive for CK8/18, a marker of epithelial cells (lilac). The upper left picture shows an overlay of CAR and mCherry fluorescence. (d) A human prostate tumor surgical sample (RPCI) was injected with AdCherry (5 × 10⁸ v.p./tumor). Twenty-four hours later, CAR (green) and mCherry (red) expression was detected in tumor epithelial cells positive for Troma I, a marker of epithelial cells (lilac). The upper left picture shows an overlay of CAR and mCherry fluorescence.