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. 2017 Oct 9;37(5):627–637. doi: 10.1038/onc.2017.369

Figure 1.

Figure 1

Alu RNA levels in CRC cells lines. (a) Northern blot analysis shows Alu RNA abundance in SW480 and SW620 cells. Densitometric values normalized against U6 small nucleolar RNA are shown in parentheses. (b) Western blot analysis shows DICER1 protein abundance in SW480 and SW620 cells. Densitometric values normalized against β-tubulin are shown in parentheses. (c) Levels of DICER1 mRNA and Alu transcripts in SW480 and SW620 as evaluated by quantitative reverse transcriptase (qRT–PCR). Transfection of DICER1 antisense oligonucleotide (DICER1 AS) downregulates DICER1 protein (d) and mRNA, and increases the abundance of Alu RNA transcripts (e) in SW480 cells as compared with control antisense oligonucleotide (Ctrl AS). Alu RNA overexpression (f) and DICER1 reduction (g) do not alter SW480 cell viability. (h) Alu RNA induces a reorganization of the cytoskeleton. Representative images of Alu RNA- (right) or vehicle- (Mock, left) transfected SW480 cells stained for F-actin with rhodamine-phalloidin (red). Nuclei are counterstained with 4',6-diamidino-2-phenylindole (DAPI, blue). Scale bar: 25 μm. For all panels: n=3; *P<0.05. Error bars denote s.e.m.