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. 2017 Oct 2;37(4):489–501. doi: 10.1038/onc.2017.352

Figure 7.

Figure 7

GHRP495T increases transcripts associated with tumour progression. BEAS-2B cells transduced with WT GHR and GHRP495T stimulated with GH (2.3 nM) for 15 min, then RNA was harvested at 60 (a) and 120 min (b) post-initial treatment. (a) Transcript levels of early GH-response genes c-FOS, EGR1, JUN at 60 min in WT GHR and GHRP495T cells normalised to B2M. (b) Transcript levels of genes associated with epithelial–mesenchymal transition (EMT) (TWIST1, SNAI2 and TGFB1), proliferation (CCND1, MYC), and elevated GHR signalling (GHR, EGFR, SOCS2 and SOCS3) at 120 min in WT GHR and GHRP495T cells normalised to B2M (see Supplementary Figure 6). Data presented as mean±s.e.m. analysed by one-way ANOVA (***P<0.001, **P<0.01, *P<0.05) and representative of three independent experiments confirmed in three independently transduced lines.