Figure 1.

Effects of Olaparib and BKM120 as single-agents or in combination on the survival and growth of PTEN-deficient endometrioid endometrial cancer cells. (a) PTEN-deficient endometrioid ensdometrial cancer cell lines as indicated were treated with drugs for 7–10 days. Fresh media with drugs were replaced every 3 days. At the end point, plates were fixed and stained with crystal violet. All experiments were performed in triplicate. Representative images of plates are shown. Error bars represent standard deviations (s.d.) from the mean. *P<0.05; **P<0.01; ***P<0.001 (Student’s t-test). (b) PTEN-deficient endometrioid endometrial cancer cell lines were treated with drugs as indicated for 24 h. Phosphorylated AKT, S6RP and 4EBP1 proteins and cleaved PARP were detected by western blot. Vinculin served as a loading control. (c) PTEN-deficient endometrioid endometrial cancer cell lines were cultured in 3D matrigel and drug treated for 10–12 days. Representative images of cells are shown in the left panel. Quantification of scored structures (intact, semi-disintegrated and disintegrated) is shown in the right panel. Scale bar, 100 μm.